10.25
Microbio-Lab
Jual Media Mikroba MRS Broth Oxoid
Telp: 087738104905
MRS BROTH (DE MAN, ROGOSA, SHARPE)
Code: CM0359
A non-selective medium for profuse
growth of `lactic acid bacteria’.
|
Typical Formula*
|
gm/litre
|
|
Peptone
|
10.0
|
|
`Lab-Lemco’ powder
|
8.0
|
|
Yeast extract
|
4.0
|
|
Glucose
|
20.0
|
|
Sorbitan mono-oleate
|
1
ml
|
|
Dipotassium hydrogen phosphate
|
2.0
|
|
Sodium acetate 3H2O
|
5.0
|
|
Triammonium citrate
|
2.0
|
|
Magnesium sulphate 7H2O
|
0.2
|
|
Manganese sulphate 4H2O
|
0.05
|
|
pH 6.2 ± 0.2 @ 25°C
|
|
*
Adjusted as required to meet performance standards
Directions
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Description
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.
MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Appearance
Dehydrated medium: Dark straw powder
Prepared medium: Amber coloured solution
Dehydrated medium: Dark straw powder
Prepared medium: Amber coloured solution
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